专利名称：GENOMIC DNA EXTRACTION REAGENT AND
METHOD
发明人：JI, Yanshan,FEI, Xiaoyin,YU,
Wenjin,MITTENDORF, Volker
申请号：EP14881909.7申请日：20141223公开号：EP3102677A1公开日：20161214
摘要：The present invention is directed to a genomic DNA extraction reagent andmethod for improved extraction of DNA from biological tissue. The extraction reagent ofthe invention is mixed with disrupted biological tissue to form a DNA extraction solutewhich is incubated in a DNA extraction step. The extraction reagent includes an alkalicomponent to maintain the DNA extraction solute at a pH of about 10 to 14 substantiallythroughout the extraction step. The extraction solute is centrifuged to clarify thesupernatant. The supernatant containing the extracted DNA is diluted with a neutralizingbuffer resulting in a high throughout method of generating high quantities of high qualityDNA. Major PCR inhibitors are managed with the unique chemical combinations of theDNA extraction reagent designed and optimized for extraction of DNA from plant tissueand cells.
申请人：Syngenta Participations AG
地址：Schwarzwaldallee 215 4058 Basel CH
国籍：CH
代理机构：Syngenta International AG
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