专利名称：Method for detection of mutant gene发明人：Tomokazu Ishikawa申请号：US12282339申请日：20070312公开号：US09234247B2公开日：20160112
摘要：Disclosed is a method for detecting mutation(s) in nucleotide sequence, whichcomprises performing a nucleic acid amplification reaction by using an oligonucleotide ora salt thereof as a primer and a nucleic acid in a sample as a template and detecting areaction product, wherein the oligonucleotide is so modified at the nucleotide at thesecond position from the 3′-terminus as to inhibit the nucleic acid synthesis. Alsodisclosed is a kit for the method. According to the present invention, since it is possibleto completely eliminate any false positive result in the determination and correspond tovarious mutation patterns by a single run of PCR1 reaction, it becomes possible to designa drug-resistance determination system, which can detect possible plural geneticmutations by a single run of multiplex PCR.
申请人：Tomokazu Ishikawa
地址：Amagasaki JP
国籍：JP
代理机构：Leydig, Voit & Mayer, Ltd.
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